Ku70 Antibody (MA5-32645) in WB Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31) (Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested.

2001-03-01

The two Ku subunits form a basket-shaped structure that threads onto the DNA end. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects. Immunogen Synthetic peptide corresponding to Human Ku70 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin. (Peptide available as ab92421) Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

Ku70

The Ku70‐specific C‐terminal region consists of a 5 kDa SAP domain (Ku70‐SAP), the structure of which was determined both in isolation (Zhang et al, 2001) and as part of the Ku70–Ku80 crystal structure (Walker et al, 2001; Fig 1C). Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation. Ku is Knockdown of Ku70 was achieved by transfecting HeLa with Ku70 specific siRNAs (Silencer® select Product # S5455, S5457). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). 2002-12-06 · Human Ku70/80 associates physically with telomerase through interaction with hTERT.

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins.

Ku70. -Ku80ヘテロ二量体と相互作用することにより非相同末端結合によるDNA 2 本鎖切断の修復を促進し，非相同末端結合にかかわるタンパク質をDNA 2本鎖

It is a heterodimeric protein made up of Ku70 and Ku80 [97] . We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. XRCC6 (X-Ray Repair Cross Complementing 6) is a Protein Coding gene.

Anti-Ku70 Antibody (E-5) is a mouse monoclonal IgG 1 κ Ku70 antibody, cited in 60 publications, provided at 200 µg/ml; raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin

We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. XRCC6 (X-Ray Repair Cross Complementing 6) is a Protein Coding gene.

a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). 2002-12-06 · Human Ku70/80 associates physically with telomerase through interaction with hTERT. Chai W(1), Ford LP, Lenertz L, Wright WE, Shay JW. Author information: (1)Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA. Ku70 is necessary and sufficient for the Ku‐FOXO4 interaction. Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27). The Ku heterodimer binds nonspecifically to DNA ends with high affinity (K d: 1–2 nM) , and FOXO4 contains a DNA binding domain required for binding Forkhead sites on DNA . 2012-06-29 · The KU70 flipper knock-out cassette was constructed with 1299bp 5′ and 1170bp 3′ flanking sequences from the KU70 locus.
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Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL). 2021-04-04 Ku70 plus Ku80 double knockout potently inhibits LPS-induced production of pro-inflammatory cytokines in human macrophages In order to exclude the possible off-target effect of the applied Ku70 and Ku80 shRNAs, we established the CRISPR/Cas9-gene editing methods to complete knockout Ku70 and Ku80. As described, the lenti- Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

In vivo, Ku70 -/- mice, known to be defective in B- but not T-lymphocyte maturation, developed thymic and disseminated T-cell lymphomas at a mean age of 6 months with CD4+/CD8+ tumor cells.
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Feb 1, 2013 Ku70 was originally described as an autoantigen, but it also functions as a DNA repair protein in the nucleus and as an antiapoptotic protein by

Supplied as 100 µL purified antibody (0.25 mg/mL). 2021-04-04 Ku70 plus Ku80 double knockout potently inhibits LPS-induced production of pro-inflammatory cytokines in human macrophages In order to exclude the possible off-target effect of the applied Ku70 and Ku80 shRNAs, we established the CRISPR/Cas9-gene editing methods to complete knockout Ku70 and Ku80. As described, the lenti- Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

Ku70 is a protein that, in humans, is encoded by the XRCC6 gene. Contents. 1 Function; 2 Aging; 3 Clinical; 4 Nomenclature; 5 Interactions; 6 References

Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects. Immunogen Synthetic peptide corresponding to Human Ku70 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin. (Peptide available as ab92421) Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Ku70 is required for late B cell development and immunoglobulin heavy chain class switching Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK).

The third research area is to study the host genetic determinants that affect HIV pathogenesis and progression. 2017-03-01 · Ku70 Antibody (G-4) is a mouse monoclonal IgG 2b (kappa light chain) provided at 200 µg/ml specific for an epitope mapping between amino acids 564-595 at the C-terminus of Ku70 of mouse origin recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF and ELISA The two characteristic KU70/KU80 functional domains were detected in both BC1G_16015.1 and BC1G_10199.1 proteins: the KU70/KU80 N-terminal α/β domain (Interpro entry IPR005161), which is similar to the von Willebrand factor type A domain (Interpro entry IPR002035), and the central KU70/KU80 β-barrel domain (Interpro entry IPR006164). Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene.